Appendix 4-A.  


(cf. Part 4)
Interpretation of Legionella Culture Results from Cooling Towers
Legionella Test Results in CFU/mLResponse
No detection (<20 CFU/mL)Maintain treatment program and Legionella monitoring in accordance with the maintenance program and plan.
For levels at ≥20 CFU/mL but <1000 CFU/mL perform the following:○ Review treatment program. ○ Institute immediate online disinfection to help with control. ○ Retest the water in 3-7 days. • Continue to retest at the same time interval until one sample retest result is < 20 CFU/mL. With receipt of result < 20 CFU/mL, resume routine maintenance program and plan. • If retest is ≥ 20 CFU/mL but < 100 CFU/mL, repeat online disinfection and retest until < 20 CFU/mL attained. • If retest is ≥100 CFU/mL but < 1000 CFU/mL, further investigate the water treatment program and immediately perform online disinfection. Retest and repeat attempts at control strategy until < 20 CFU/mL attained. ○ If retest is ≥ 1000 CFU/mL, undertake control strategy as noted below.
For levels ≥1000 CFU/mL perform the following:○ Review the treatment program and provide appropriate notifications per section 4-1.6 of this Subpart. ○ Institute immediate online decontamination to help with control. ○ Retest the water in 3-7 days. • Continue to retest at the same time interval until one sample retest result is < 20 CFU/mL. With receipt of result < 20 CFU/mL, resume routine maintenance program and plan. • If any retest is ≥ 20 CFU/mL but < 100 CFU/mL, repeat online disinfection and retest until < 20 CFU/mL attained. • If any retest is ≥ 100 CFU/mL but < 1000 CFU/mL, further investigate the water treatment program and immediately perform online disinfection. Re-test and repeat attempts at control strategy until < 20 CFU/mL attained. • If any retest is ≥ 1000 CFU/mL: • carry out system decontamination
Colony forming units per milliliter.
Online disinfection means – Dose the cooling tower water system with either a different biocide or a similar biocide at an increased concentration than currently used.
Online decontamination means – Dose the recirculation water with a halogen-based compound (chlorine or bromine) equivalent to at least 5 milligrams per liter (mg/L) or parts per million (ppm) free residual halogen for at least one hour.
System decontamination means – Maintain between 5 to 10 mg/L (ppm) free residual halogen for a minimum of one hour; drain and flush with disinfected water; clean wetted surface; refill and dose to 1 – 5 mg/L (ppm) of free residual halogen and circulate for 30 minutes. Refill, re-establish treatment and retest for verification of treatment.
For chlorine treatment the pH range should be 7.0 to 7.6; for bromine treatment the pH range should be 7.0 to 8.7. At higher pH values the treatment times may need to be extended.
NOTE: Stabilized halogen products should not be used for online decontamination or system decontamination as defined in this Appendix per footnotes 3 and 4.